CLUSTAL O(1.2.4) multiple sequence alignment NL63 --MKLFLILLVLPLASCFFTCNSNANLS-MLQLGVPDNSSTIVTGLLPTHWFCA----NQ 53 MERS MIHSVFLLMFLLTPTESYVDVGPDSVKSACIE-------VDIQQTFFDKTWPRP-IDVSK 52 SARS-CoV-2 ----MFVFLVLLPLV-S----------SQCVN---------LTT--RTQLPPAY--TNSF 32 SARS ----MFIFLLFLTLT-S----------GSDLD---------RCTTFDDVQAPNYTQHTSS 36 :*:::..* . . . :: . NL63 STSVYSA------------NGFFYIDVGNHRSAFALHTGYYDANQYYIYVTNEIGLNASV 101 MERS ADGIIYPQGRTYSNITITYQGLF-PYQGDHGDMYVYSAGHA------------TGTTPQK 99 SARS-CoV-2 TRGVYYPDKVFRSSVLHSTQDLFLPFFSNVT---WFHAIHV------------SGTNGTK 77 SARS MRGVYYPDEIFRSDTLYLTQDLFLPFYSNVT---GFHTIN-------------------H 74 .: :.:* .: : NL63 T------LKICKFSRNTTFDFLSNASSS---------FDCIVNLLFTEQLGAPLGITISG 146 MERS LFVANYSQDVKQFANGFVVRIGAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDG 159 SARS-CoV-2 R----FDNPVLPFNDGVYFASTE-----------------KSNIIRGWIFGTTLDSKTQ- 115 SARS T----FGNPVIPFKDGIYFAATE-----------------KSNVVRGWVFGSTMNNKSQ- 112 : * . . :: :*: :. . NL63 ETVRLHLYNVTRTFYVPAAYKLTKLSVKCYFNYSCVFSVVNATVTVNVTTHNGRVVNYTV 206 MERS KMGR-------------------------FFNHTLVLL-PDG------CGTLLRAF--YC 185 SARS-CoV-2 ---------------------------------SLLIV-NNA------TNVVIKVCEFQF 135 SARS ---------------------------------SVIII-NNS------TNVVIRACNFEL 132 : :: :. :. NL63 CDDC---------NGYTDNIFSVQQDGRIPNGFPFNNWFLLTNGST---LVDGVSRLYQP 254 MERS ILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNI 245 SARS-CoV-2 CNDPFLGVYYHKNN---------------------KSWMESEFRVYSSANNCTFEYVSQP 174 SARS CDNPFFAVSKPMGT-------------------------QTHTMIFDNAFNCTFEYISDA 167 : . . : NL63 LRLTCLWPVPGLKSSTGFVYFNATGSDVNCNGYQHNSVVDVMRYNLNFSANSLDNLKSGV 314 MERS TEDEILEWFGITQTAQG-VHLFSSRYVDLYGGN--------------------------- 277 SARS-CoV-2 FLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINL--------------VRDLPQGF 220 SARS FSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDV--------------VRDLPSGF 213 : *. NL63 IVFKTLQYDVLFYCSNSSSGVLDTTIPFGPSSQPYYCFINSTINTTHVSTFVGILPPTVR 374 MERS --MFQF-----------------ATLPVYDTIK-----------------YYSIIPHSIR 301 SARS-CoV-2 SALEPL-----------------VDLPIGINIT-----------------RFQTLLALHR 246 SARS NTLKPI-----------------FKLPLGINIT-----------------NFRAILTAFS 239 : : :*. . : NL63 EIVVARTGQFYINGFKYFDLGFIEAVNFNVTTASATDFWTVAFATFVDV-------LVN- 426 MERS ------------------------------SIQSDRKAW----AAFYVYKLQPLTFLLDF 327 SARS-CoV-2 ---------------------------SYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKY 279 SARS ---------------------------------PAQDIWGTSAAAYFVGYLKPTTFMLKY 266 . * *:: ::. NL63 VSATNIQNLLY-CDSPFEKLQCEHLQFGLQDGFYSANFLDDNVL------PETYV----- 474 MERS SVDGYIRRAIDCGFNDLSQLHCSYESFDVESGVYSVSSFEAKPSGSVVEQAEG-VECDFS 386 SARS-CoV-2 NENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFG 339 SARS DENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFG 326 * : . : : :* .* ::.*.*... : : NL63 ------ALPIYYQHTDINFTA-----------TASFGGSCYVCKPHQVNISLNGNTSVCV 517 MERS PLLSG-TPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIA-------SNCY 438 SARS-CoV-2 EVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLN-------DLCF 392 SARS EVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLN-------DLCF 379 * :: .* . : . * NL63 R---TSHFSIRYIY-NRVKSGSPGD---SSWHIYLKSGTCPFSFS--------------- 555 MERS SSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYS 498 SARS-CoV-2 TNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYL 452 SARS SNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYK 439 . * : .. * .:: . * : . NL63 -------KLNNFQK-FKTICFSTVEVPGSCNFPLEATWHYTSY--------------TIV 593 MERS YINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPST-VWEDGDYYRKQLSPLEGGGWLVAS 557 SARS-CoV-2 YRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYF-----------PLQSYGFQPTN 501 SARS YRYLRHGKLRPFERDISNVPFSPDGKPCTP-PALNCYW-----------PLNDYGFYTTT 487 * . . * : NL63 GALY-------VTWSEGNSITGVPYPVSGI-----REFSNLVLNNCTKYNIYDYVGTGII 641 MERS GSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYSLYGVSGRGVF 617 SARS-CoV-2 GVGYQPYRVVVLSFELL----HAPATVCGP-----KKSTNLVKNKCVNFNFNGLTGTGVL 552 SARS GIGYQPYRVVVLSFELL----NAPATVCGP-----KLSTDLIKNQCVNFNFNGLTGTGVL 538 * : : *. .:*.::.: . * *:: NL63 RSSNQSLAGGITYVSNSGN-L-LGFKNVSTGNIFIVTPCNQPDQVAVYQ----------- 688 MERS QNCTAVGVRQQRFVYDAYQNLVGYYSD--DGNYYCLRACVSVPVSVIYD--KETKTHATL 673 SARS-CoV-2 TESNKKFLPFQQFGRDIADTT-DAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVL 611 SARS TPSSKRFQPFQQFGRDVSDFT-DSVRDPKTSEILDISPCAFGGVSVITPGTNASSEVAVL 597 .. : : : : : : * .: NL63 -QSIIGAMTAVNESRYGLQNLLQLPNFYYVSNG------GNNCTTAVMT----------Y 731 MERS FGSVACEHISSTMSQYSRS---TRSMLKRRDSTYGPLQTPVGCVLGLVNSSLFVEDCKLP 730 SARS-CoV-2 YQDVNCTEVPVAIHADQLT-----PTWRVYSTGSNVFQTRAGCLIGAEHVN-NSYECDIP 665 SARS YQDVNCTDVSTAIHADQLT-----PAWRIYSTGNNVFQTQAGCLIGAEHVD-TSYECDIP 651 .: .. .* . NL63 SNFGICADGS----LIPVRPRNSSDNGI---------------SAIITANLSIPSNWTTS 772 MERS LGQSLCALPDTPSTLTPRSVRSVPGEMRLASIAFNHPIQV-DQLNSSYFKLSIPTNFSFG 789 SARS-CoV-2 IGAGICASYQTQT-NSPRRARSVASQSI---IAYTMSLGAENSVAYSNNSIAIPTNFTIS 721 SARS IGAGICASYHTVS-L----LRSTSQKSI---VAYTMSLGADSSIAYSNNTIAIPTNFSIS 703 . .:** *. : .::**:*:: . NL63 VQVEYLQITSTPIVVDCATYVCNGNPRCKNLLKQYTSACKTIEDALRLSAHLETNDVSSM 832 MERS VTQEYIQTTIQKVTVDCKQYVCNGFQKCEQLLREYGQFCSKINQALHGANLRQDDSVRNL 849 SARS-CoV-2 VTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEV 781 SARS ITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREV 763 : * : : *** *:*.. .* :** :* . *. :: ** : .. .: NL63 LTFDSNAFSLANVTSFGDY-NLSSVLPQRNIRSSRIAGRSALEDLLFSKVVTSGLGTVDV 891 MERS FASVKSSQSSPIIPGFGGDFNLTLLEPVS-ISTGSRSARSAIEDLLFDKVTIADPGYMQ- 907 SARS-CoV-2 FAQVKQIYKTPPIKDFGGF-NFSQILPDP----SKPSKRSFIEDLLFNKVTLADAGFIK- 835 SARS FAQVKQMYKTPTLKYFGGF-NFSQILPDP----LKPTKRSFIEDLLFNKVTLADAGFMK- 817 :: .. . : **. *:: : * : ** :*****.**. :. * :. NL63 DYKSCT--KGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLIGGMVLGG----LTSA 945 MERS GYDDCMQQGPASARDLICAQYVAGYKVLPPLMDVNMEAAYTSSLLGSIAGVGWTAGLSSF 967 SARS-CoV-2 QYGDCL--GDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAA 893 SARS QYGECL--GDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAA 875 * .* ** *** * *** : : * **.:*:.. * : NL63 AAIPFSLALQARLNYVALQTDVLQENQKILAASFNKAINNIVASFSSVNDAITQTAEAIH 1005 MERS AAIPFAQSIFYRLNGVGITQQVLSENQKLIANKFNQALGAMQTGFTTTN----------- 1016 SARS-CoV-2 LQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTA----------- 942 SARS LQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTS----------- 924 ***: : *:* :.: :** **** :* .**.*:. : .:::. NL63 TVTIALNKIQDVVNQQGSALNHLTSQLRHNFQAISNSIQAIYDRLDSIQADQQVDRLITG 1065 MERS ---EAFQKVQDAVNNNAQALSKLASELSNTFGAISASIGDIIQRLDVLEQDAQIDRLING 1073 SARS-CoV-2 ---SALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITG 999 SARS ---TALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITG 981 *: *:**.**::..**. *..:* .* *** : * .*** :: : *:****.* NL63 RLAALNAFVSQVLNKYTEVRGSRRLAQQKINECVKSQSNRYGFCGNGTHIFSIVNSAPDG 1125 MERS RLTTLNAFVAQQLVRSESAALSAQLAKDKVNECVKAQSKRSGFCGQGTHIVSFVVNAPNG 1133 SARS-CoV-2 RLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHG 1059 SARS RLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHG 1041 ** :*:::*:* * : . * .** *:.*** .**:* .***:* *:.*: **.* NL63 LLFLHTVLLPTDYKNVKAWSGICVDGIYGYVLRQPNLVLYSD-----NGVFRVTSRIMFQ 1180 MERS LYFMHVGYYPSNHIEVVSAYGLCDAANPTNCIAPVNGYFIKTNNTRIVDEWSYTGSSFYA 1193 SARS-CoV-2 VVFLHVTYVPAQEKNFTTAPAICHDGKA---HFPREGVFVSN-----GTHWFVTQRNFYE 1111 SARS VVFLHVTYVPSQERNFTTAPAICHEGKA---YFPREGVFVFN-----GTSWFITQRNFFS 1093 : *:*. *:: :. : .:* . : : : * :: NL63 PRLPVLSDFVQIYNCNVTFVNISRVELHTVIPDYVDVNKTLQEFAQNLPKYVKPNFDLTP 1240 MERS PEPITSLNTKYVAPQVTY-QN-----ISTNLPPPLLGNSTGIDFQDELDEFFKNVSTSIP 1247 SARS-CoV-2 PQIITTDNTFVSGNCDVV-IGI----VNNTVYDPLQ--PELDSFKEELDKYFKNHTSPDV 1164 SARS PQIITTDNTFVSGNCDVV-IGI----INNTVYDPLQ--PELDSFKEELDKYFKNHTSPDV 1146 *. . : . . : . : : .* ::* ::.* NL63 FNLTYLNLSSELKQLEAKTASLFQTTVELQGLIDQINSTYVDLKLLNRFENYIKWPWWVW 1300 MERS N-------FGSLTQINTTLLDLTYEMLSLQQVVKALNESYIDLKELGNYTYYNKWPWYIW 1300 SARS-CoV-2 D-------LGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIW 1217 SARS D-------LGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVW 1199 ..:. :::. .: *: : . :*.: :**: *..: * ****::* NL63 LIISVVFVVLLSLLVFCCLSTGCCGCCNCLTSSMRGCCDCGSTKLPYYEFEKVH----VQ 1356 MERS LGFIAGLVALALCVFFILCCTGCGTNC-------MGKLKCNRCCDRYEEYDLEPHKVHVH 1353 SARS-CoV-2 LGFIAGLIAIVMVTIMLCCMTSCCSCL-------KGCCSCGSCCKF-DEDDSEPVLKGVK 1269 SARS LGFIAGLIAIVMVTILLCCMTSCCSCL-------KGACSCGSCCKF-DEDDSEPVLKGVK 1251 * : . ::.: .: *.* * .*. * : *: NL63 ---- 1356 MERS ---- 1353 SARS-CoV-2 LHYT 1273 SARS LHYT 1255