LOCUS Exported 3815 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUC57-Ins2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3815) AUTHORS Stephane Emond TITLE Direct Submission JOURNAL Exported Wednesday, May 12, 2021 from SnapGene Viewer 5.2.4 https://www.snapgene.com FEATURES Location/Qualifiers source 1..3815 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 6..11 /label=AcuI /note="/vntifkey=21" misc_feature 28..33 /label=N6 insert /label=N6\insert /note="/vntifkey=21" misc_feature 39..43 /label=MlyI /note="/vntifkey=21" protein_bind 84..104 /label=Protein Bind 1 /label=Protein_Bind_1 /bound_moiety="lacI" /note="lac repressor" /note="/vntifkey=31" protein_bind 151..188 /label=Protein Bind 2 /label=Protein_Bind_2 /bound_moiety="CAP" /note="/vntifkey=31" misc_feature 421..441 /label=Rep-B /note="/vntifkey=21" misc_feature 449..1063 /label=Misc Feature 1 /label=Misc_Feature_1 /note="replicon of pMB1" /note="/vntifkey=21" rep_origin 463 /label=Rep Origin 1 /label=Rep_Origin_1 /note="/vntifkey=33" CDS complement(1223..2083) /codon_start=1 /transl_table=11 /gene="bla" /product="beta-lactamase" /function="confers resistance to ampicilin" /label=bla /note="/vntifkey=4" /db_xref="GI:4379389" /db_xref="InterPro:IPR000871" /db_xref="InterPro:IPR001466" /db_xref="InterPro:IPR012338" /protein_id="CAA75111.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene complement(1223..2083) /gene="bla" /label=bla /note="/vntifkey=60" mat_peptide 1226..2014 /gene="bla" /product="beta-lactamase" /label=bla /note="/vntifkey=84" sig_peptide 2015..2083 /gene="bla" /label=bla /note="/vntifkey=94" misc_feature 2687..2689 /label=N2N3N4 /note="/vntifkey=21" misc_feature 2690 /label=Cut position /label=Cut\position /note="/vntifkey=21" misc_feature join(2694..3815,1..33) /label=Ins2 misc_feature 2706..2711 /label=AcuI /note="/vntifkey=21" terminator 2726..2826 /label=t0 /note="/vntifkey=43" CDS complement(2841..3632) /codon_start=1 /label=KanR /note="/vntifkey=4" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 catggctgaa ggccactcga gatcgatnnn nnngcgctga ctcaagcttg gcgtaatcat 61 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 121 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 181 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 241 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 301 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 361 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 421 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 481 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 541 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 601 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 661 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 721 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 781 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 841 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 901 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 961 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 1021 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 1081 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 1141 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 1201 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 1261 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 1321 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 1381 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 1441 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 1501 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 1561 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 1621 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 1681 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 1741 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 1801 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 1861 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 1921 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 1981 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 2041 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 2101 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 2161 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 2221 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 2281 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 2341 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 2401 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag 2461 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 2521 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 2581 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 2641 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attctagatc tgcggccgca 2701 tccgtcttca gtcgctgctg actagtgctt ggattctcac caataaaaaa cgcccggcgg 2761 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac 2821 agaaagcgag ctcgatatca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 2881 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 2941 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc 3001 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 3061 gcatcgccgt gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 3121 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 3181 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 3241 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 3301 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 3361 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 3421 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 3481 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 3541 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agctgccgga 3601 gaacctgcgt gcaatccatc ttgttcaatc attttagctt ccttagctcc tgaaaatctc 3661 gacaactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct 3721 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca 3781 acagggacac caggatttat ttattctgcg aagtc //