LOCUS       Exported                3818 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pUC57-Ins3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Stephane Emond
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 12, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    6..11
                     /label=AcuI
                     /note="/vntifkey=21"
     misc_feature    28..36
                     /label=N9 insert
                     /label=N9\insert
                     /note="/vntifkey=21"
     misc_feature    42..46
                     /label=MlyI
                     /note="/vntifkey=21"
     protein_bind    87..107
                     /label=Protein Bind 1
                     /label=Protein_Bind_1
                     /bound_moiety="lacI"
                     /note="lac repressor"
                     /note="/vntifkey=31"
     protein_bind    154..191
                     /label=Protein Bind 2
                     /label=Protein_Bind_2
                     /bound_moiety="CAP"
                     /note="/vntifkey=31"
     misc_feature    424..444
                     /label=Rep-B
                     /note="/vntifkey=21"
     misc_feature    452..1066
                     /label=Misc Feature 1
                     /label=Misc_Feature_1
                     /note="replicon of pMB1"
                     /note="/vntifkey=21"
     rep_origin      466
                     /label=Rep Origin 1
                     /label=Rep_Origin_1
                     /note="/vntifkey=33"
     CDS             complement(1226..2086)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /note="/vntifkey=4"
                     /db_xref="GI:4379389"
                     /db_xref="InterPro:IPR000871"
                     /db_xref="InterPro:IPR001466"
                     /db_xref="InterPro:IPR012338"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1226..2086)
                     /gene="bla"
                     /label=bla
                     /note="/vntifkey=60"
     mat_peptide     1229..2017
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="/vntifkey=84"
     sig_peptide     2018..2086
                     /gene="bla"
                     /label=bla
                     /note="/vntifkey=94"
     misc_feature    2690..2692
                     /label=N2N3N4
                     /note="/vntifkey=21"
     misc_feature    2693
                     /label=Cut position
                     /label=Cut\position
                     /note="/vntifkey=21"
     misc_feature    join(2697..3818,1..36)
                     /label=Ins3
     misc_feature    2709..2714
                     /label=AcuI
                     /note="/vntifkey=21"
     terminator      2729..2829
                     /label=t0
                     /note="/vntifkey=43"
     CDS             complement(2844..3635)
                     /codon_start=1
                     /label=KanR
                     /note="/vntifkey=4"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 catggctgaa ggccactcga gatcgatnnn nnnnnngcgc tgactcaagc ttggcgtaat
       61 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      121 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      181 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      241 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      301 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      361 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      421 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      481 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      541 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      601 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      661 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      721 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      781 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      841 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      901 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      961 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1021 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1081 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1141 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1201 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1261 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1321 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1381 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1441 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1501 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     1561 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1621 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1681 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1741 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     1801 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     1861 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     1921 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     1981 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2041 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2101 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2161 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     2221 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     2281 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     2341 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     2401 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     2461 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     2521 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     2581 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     2641 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattctag atctgcggcc
     2701 gcatccgtct tcagtcgctg ctgactagtg cttggattct caccaataaa aaacgcccgg
     2761 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2821 aacagaaagc gagctcgata tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     2881 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     2941 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     3001 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     3061 caggcatcgc cgtgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     3121 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     3181 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     3241 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     3301 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     3361 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     3421 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     3481 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     3541 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagctgcc
     3601 ggagaacctg cgtgcaatcc atcttgttca atcattttag cttccttagc tcctgaaaat
     3661 ctcgacaact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     3721 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
     3781 tcaacaggga caccaggatt tatttattct gcgaagtc
//
