LOCUS       Exported                3763 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pUC57-Del2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Stephane Emond
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 12, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      5..105
                     /label=t0
                     /note="/vntifkey=43"
     CDS             complement(120..911)
                     /codon_start=1
                     /label=KanR
                     /note="/vntifkey=4"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    1099..1103
                     /label=MlyI
                     /note="/vntifkey=21"
     misc_feature    1109..1111
                     /label=Triplet4
                     /note="/vntifkey=21"
     misc_feature    1112..1114
                     /label=Triplet5
                     /note="/vntifkey=21"
     protein_bind    1152..1172
                     /label=Protein Bind 1
                     /label=Protein_Bind_1
                     /bound_moiety="lacI"
                     /note="lac repressor"
                     /note="/vntifkey=31"
     protein_bind    1219..1256
                     /label=Protein Bind 2
                     /label=Protein_Bind_2
                     /bound_moiety="CAP"
                     /note="/vntifkey=31"
     misc_feature    1489..1509
                     /label=Rep-B
                     /note="/vntifkey=21"
     misc_feature    1517..2131
                     /label=Misc Feature 1
                     /label=Misc_Feature_1
                     /note="replicon of pMB1"
                     /note="/vntifkey=21"
     rep_origin      1531
                     /label=Rep Origin 1
                     /label=Rep_Origin_1
                     /note="/vntifkey=33"
     CDS             complement(2291..3151)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /note="/vntifkey=4"
                     /db_xref="GI:4379389"
                     /db_xref="InterPro:IPR000871"
                     /db_xref="InterPro:IPR001466"
                     /db_xref="InterPro:IPR012338"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2291..3151)
                     /gene="bla"
                     /label=bla
                     /note="/vntifkey=60"
     mat_peptide     2294..3082
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="/vntifkey=84"
     sig_peptide     3083..3151
                     /gene="bla"
                     /label=bla
                     /note="/vntifkey=94"
     misc_feature    3749..3751
                     /label=Triplet1
                     /note="/vntifkey=21"
     misc_feature    3752..3754
                     /label=Triplet2
                     /note="/vntifkey=21"
     misc_feature    join(3755..3763,1..1108)
                     /label=Del2
     misc_feature    3757..3761
                     /label=MlyI
                     /note="/vntifkey=21"
ORIGIN
        1 ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
       61 tccagatgga gttctgaggt cattactgga tctatcaaca gaaagcgagc tcgatatcag
      121 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
      181 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
      241 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
      301 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccgtg ggtcacgacg
      361 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
      421 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
      481 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
      541 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
      601 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
      661 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
      721 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
      781 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
      841 cagtcatagc cgaatagcct ctccacccaa gctgccggag aacctgcgtg caatccatct
      901 tgttcaatca ttttagcttc cttagctcct gaaaatctcg acaactcaaa aaatacgccc
      961 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct
     1021 cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt
     1081 tattctgcga agtccatgga gtcatcccgg gaagcttggc gtaatcatgg tcatagctgt
     1141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1261 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1321 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1381 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1981 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2041 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2221 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2281 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2341 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2401 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2461 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2521 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2581 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2641 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2701 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2761 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2821 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2881 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2941 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3001 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3061 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3121 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3181 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3241 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     3301 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     3361 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     3421 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     3481 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     3541 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     3601 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     3661 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     3721 cacgacgttg taaaacgacg gccagtgaat tcccgggact cga
//
